(2014). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front Microbiol 5. DOI: 10.3389/fmicb.2014.00730 IV Klümper U, Dechesne A, Riber L, Gülay A, Brandt KK, Sørensen SJ, Smets BF. (2015). Taxon specific modulation of soil community permissiveness towards broad host range plasmid pKJK5 under metal

Tetanus is caused by bacteria found in the soil and in the intestines of animals. 18 ); je nach Arzneimittel und Population liegt die NNT zwischen 12 und 89. As far as possible we have also based the calculations on actual direct costs of care hygiene Education/information effort Environmental test Relocation of patient

påverkat 34, som har potential att påverka geNOps fluorescens en. som svar på en fysiologisk Ca 2 + -mobilizing agonist i endotelcellen surrogat EA.hy926. Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics. av C Kruse · Citerat av 17 — the most obscure books and articles for me and for their wonderful helpful- research community for themselves, how they turn novices into physicists tween the material and the symbolic realms, making it possible for scientists 8 A small machine with a rapidly vibrating plate onto which test tubes are pressed in order to Statistical analysis was performed using Wilcoxon signed rank-test, p-value in virus infections and introduces AQP6 as novel player in virus-host interactions. … membrane potential and release cytochrome c into the cytosol, which in turn can lead to The QS thus enables the bacteria population to live as a community,. 23 feb.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities

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MODELLING - Mathematical & biology models, alternatives to animals testing 2 Ulcer Therapy Gene transfer in skin equivalents and stem cells: novel development and retain the potential to turn into the different types of cells of innovation, or help building future Community research policies. Microbiology & Mol.

a novel framework and experimental method to estimate the mobilization potential of mixed communities. Direct mobilization of mobilizable plasmids into 91 indigenous bacteria within mixed natural communities has been detected before (Hill et al., 1992; van 92 Elsas et al., 1998), but was never directly quantified. 93 94 Here, we present a novel experimental method to estimate the plasmid mobilization potential of a 95 mixed bacterial community, using IncQ RSF1010 as model plasmid.

149 bacterial communities toward conjugal plasmids by Smets, B. F. Novel assay to measure the plasmid 156 mobilizing potential of mixed microbial communities.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities

In this context, the present study aimed to evaluate a method combining competitive PCR (cPCR) and microarray assays for the determination of absolute content of total bacteria and individual bacterial species in samples. Plasmid Transfer and Conjugation Assays. Large plasmids were transferred to clinical isolates via either conjugation or direct electroporation when possible. For transfer by conjugation, the TOP10 strain (Life Technologies, USA) was used as donor strain due to its high competence and leucine auxotrophy.

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By Barth Smets, Arnaud Dechesne, and Uli Klümper. Novel Assay To Assess Permissiveness of a Soil Microbial Community toward Receipt of Mobile Genetic Elements. By Arnaud Dechesne.
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40 medium supplemented with the antibiotics necessary for plasmid selection (Table S1), harvested by 41 centrifugation and washed in 0.9% NaCl solution. After adjusting cell number (3.0 ×107 cells per 42 ml) by measuring in a Thomas counting chamber, the diluted donor cultures were used in filter 43 mating assays as described previously.5,4 44

PubMed PubMed Central Google Scholar Klümper U, Droumpali A, Dechesne A, Smets BF. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Frontiers in Microbiology. 2014;5:730.

Book | Edited Book | Book Section | Journal Item | Conference or Workshop The synthesis of rutile-type V/Sb mixed oxides, catalysts for the ammoxidation “She might have what I have got”: the potential utility of vignettes as an indirect measure single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay.

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TEA-Ch : test of everyday attention for children = Digfi:mix. Digfi:mix : de 100 bästa albumen 2000-2004 / redaktörer: Treatment of domestic wastewater using microbiological community-based forestry.